EVAg

During visit of the facility examples for comprehensive detection and measures to control quarantine pathogens (viruses, bacteria, fungi, nematodes) will be presented and the associated quality management will be demonstrated. Furthermore time for mutual scientific exchange and discussions on the control of known and emerging viruses is given. The number of applicants is limited to one to two persons per visiting period.

High-throughput sequencing has developed into a versatile standard tool in plant virology research. It provides fast and precise genomic data on the viruses being processed for a wide range of applied and basic research questions. This service package offers the individual analysis of three plant samples by HTS to ensure a reliable detection and assembly of the viral genomes.

Menangle virus N protein antiserum (Rabbit)

Purified plasmids with: S segment of Mopeia virus, L segment of Mopeia Virus, NP Expression plasmid, L expression plasmid, all under T7 Polymerase promotor; 100 ng per Plasmid, shipped at RT. Rescue of virus requires cell line expressing T7 Polymease (e.g. BSR-T7 cells) or co-transfection of a T7 polymerase plasmid; cells are not included in this bundle

Plasmid coding the full-length antigenome of Ebola virus isolate Makona from West African outbreak 2014. The plasmid can be used for the rescue of recombinat virus.

Plasmid coding the full-length antigenome of Ebola virus Zaire isolate Mayinga (AF086833) . The plasmid can be used for the rescue of recombinat virus.

Plasmid encoding the full-length anti-genome of Marburg virus strain Musoke under the control of the T7 polymerase for rescue experiments. The plasmid contains two genetic markers: insertion of a SacII restriction site in the GP gene (nt position 10511a to g silent mutation) and a silent mutation at nt position 9485 c toT in the GP gene resulting in the deletion of a KpnI restriction site

RNA extracted from infected tissue, Oropouche virus (RIVM/OROV/TRVL9760/09/11/1967).

Full length Hendra virus genome clone to produce recombinant virus

Full length Nipah virus Bangladesh genome clone to produce recombinant virus

Purified recombinant NS1 protein (C-terminal His-Tagged) expressed in mammalian HEK293F cells as secreted protein

WEN3; LCMV-WE GP neutralizing antibody, monoclonal antibody, mouse IgG2a

Galinsoga mosaic virus full-length cDNA is cloned in plasmid pUC18, under the control of a T7 promoter, allowing for transcription of infectious RNA

Tomato bushy stunt virus full-length cDNA is cloned in plasmid pUC18, under the control of a T7 promoter, allowing for transcription of infectious RNA

Tula virus RNA isolated from naturally infected Microtus agrestis

The full-length genome of BYDV-PAV is cloned in the binary plasmid vector pBin61, allowing transformation into Agrobacterium and infection of plants

Newcastle disease virus (NDV V4 Q91) monoclonal antibody (IgG2a)

Rabbit Haemorrhagic Disease Virus 2, RHDV-2 RNA extract. Extracted using the MagMAX™ CORE Nucleic Acid Purification Kit. Isolated from wild rabbit.

Protein A chromatography purified Watermelon mosaic virus (WMV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Turnip yellow mosaic virus (TYMV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Turnip ringspot virus (TuRSV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Turnip mosaic virus (TuMV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Tomato bushy stunt virus (TBSV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Tobacco rattle virus (TRV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Southern bean mosaic virus (SBMV) polyclonal antibodies, raised in rabbit, suitable for serological detection