In order to assess the quality of EVAg viruses and viral products, a set of inscription criteria for EVAg products has been reviewed and developed.
EVAg Virus products
3 categories of Human/Animal virus products :
Platinum represents the highest quality standards of our viruses. It contains all the viruses that meet the following criteria:
Freeze dried or frozen
complete coding sequence or complete genomic sequence
mycoplasmae free
infectivity quantified (ID50 or pfu), except for non cultivable virus samples for which infectivity cannot be tested
Gold viruses are:
Frozen
complete coding sequence or complete genomic sequence
mycoplasma tested (whatever the result)
infectivity tested (not quantified)
Silver viruses represents <25% of strains entered into the EVAg catalogue (Portal).
They are viruses that have incomplete but significant molecular characterisation (Partial sequence) or viruses for which the test for mycoplasmic content was not performed.
3 categories of Plant virus products:
Platinum category contains all the viruses that meet the following criteria:
complete coding sequence or complete genomic sequence
contamination with co-infecting virus tested and negative
Gold viruses are:
complete coding sequence or complete genomic sequence
contamination with co-infecting virus tested and positive
Silver plant viruses are viruses that have incomplete but significant molecular characterisation (Partial sequence) or viruses for which the test for contamination with co-infecting virus was not performed.
EVAg cloned nucleic acids
4 categories of cloned nucleic acids :
Platinum represents the cloned nucleic acids that meet the following criteria:
cDNA fully sequenced and checked
No mutation compared to reference sequence
Gold cloned nucleic acids have:
cDNA fully sequenced and checked
Mutations mentioned (no frameshift, no unexpected STOP codon accepted)
Silver contains all nucleic acids that meet the following criteria:
cDNA partly sequenced and checked
no mutation compared to reference sequence
Bronze gathers cloned nucleic acids that have:
cDNA partly sequenced and checked
Mutations mentioned (no frameshift, no unexpected STOP codon accepted)
EVAg antibodies
4 categories of antibodies :
Platinum represents the highest quality standards of EVAg antibodies. Antibodies in this category are:
Monoclonal
Purified by affinity
Specificity documented
Characterised by at least 3 out of the 5 following methods : Western Blot, ELISA, IFA, neutralisation, immunoprecipitation
Gold antibodies are:
Monoclonal
Specificity documented
Characterised by at least 2 out of the 5 following methods : Western Blot, ELISA, IFA, neutralisation, immunoprecipitation
Silver contains all antibodies that meet the following criteria:
Monoclonal
Specificity not documented
Characterised by at least 1 out of the 5 following methods : Western Blot, ELISA, IFA, neutralisation, immunoprecipitation
Bronze gathers antibodies that are:
Seral
or Polyclonal antibodies
or Uncharacterised monoclonal antibodies
EVAg proteins
3 categories of proteins:
Platinum proteins meet the following criteria:
Purified (> 95%)
functionally characterised (enzymatic or antigenic, or by Mass Spec)
Gold contains all proteins that meet the following criteria:
Purified (> 95%)
NO functional characterisation
Silver proteins are the unpurified proteins or partly purified proteins (expression host lysate, presence of the protein assessed by immunoassay)