Product Description
Ref-SKU:
016N-05982 Plasmid coding the full-length antigenome of Ebola virus Zaire isolate Mayinga (AF086833) . The plasmid can be used for the rescue of recombinat virus.
Product Risk Group:
Information on the related virus
ICTV Taxonomy:
Virus name:
Subspecies:
Isolate:
Cloned Product: Yes
Can it be used to produce GMO:
Yes
Biosafety restrictions:
The plasmid can be used to rescue recombinant RG4 virus, it will be provided only to operating BSL4 laboratories
Region encompassed in this Product:
full-lenght antigenome of Ebola virus isolate Mayinga
Plasmid selection:
TAG:
Sequencing:
Fully sequenced
Sequence checked:
Yes
Mutations:
Contain mutations (no frameshift, no unexpected STOP codon)
Observed mutations:
introduced genetic marker GP gene ORF (G7252A) silent mutation and in the VP30 gene an XmaI restriction site was inserted
Genbank reference:
Identification technique:
sequencing and enzymatic restriction
Nucleic Acid preparation technique:
Qiagen Maxi-prep kit
Storage conditions:
TE Buffer -20C
Shipping conditions:
IATA Classification:
Virus host type:
Information about the origin of the product
Recombinant product:
This product is a recombinant product
Biological material origin:
Synthetic origin & Natural origin
Natural part identification: sequence of Ebola virus Zaire isolate Mayinga
Natural part identification:
sequence of Ebola virus Zaire isolate Mayinga
Genbank reference:
Collection date: BEFORE
Thursday, 13 December, 2012
Country of collection:
Synthetic part identification: pAmp minimal vector published in doi:10.1371/journal.ppat.1002847
Synthetic part identification:
pAmp minimal vector published in doi:10.1371/journal.ppat.1002847
Genbank reference:
Modifications from the reference sequence(s):
The sequence(s) of this synthetic product has been modified from the sequence listing provided as Genbank references
Description of modification(s) made from the reference sequence(s):
doi:10.1371/journal.ppat.1002847