EVAg

Virus whole genome sequencing

An in-depth theoretical and practical application of biosafety, biocontainment and biosecurity under the Australia/New Zealand standards and Australian regulatory framework for Biosecurity Containment and Physical Containment level 3 and 4 laboratory and animal facilities.

Lyophilized encapsidated RNA (ArRNA) contains a region of the MS2 phage and is used as positive control for extraction and real time RT-PCR. The ArRNA is especially developed to be spiked in nasopharyngeal, salivary, urine and stool samples. Extraction is mandatory before use in real time RT-PCR. Packaged for 100 Rxns, for RUO (Research Use Only). SOPs in the attached pdf.

Lyophilized encapsidated RNA (ArRNA) contains a region of the MS2 phage and is used as positive control for extraction and real time RT-PCR. The ArRNA is especially developed to be spiked in whole blood and serum samples. Extraction is mandatory before use in real time RT-PCR. Packaged for 100 Rxns, for RUO (Research Use Only). SOPs in the attached pdf.

During visit of the facility examples for comprehensive detection and measures to control quarantine pathogens (viruses, bacteria, fungi, nematodes) will be presented and the associated quality management will be demonstrated. Furthermore time for mutual scientific exchange and discussions on the control of known and emerging viruses is given. The number of applicants is limited to one to two persons per visiting period.

High-throughput sequencing has developed into a versatile standard tool in plant virology research. It provides fast and precise genomic data on the viruses being processed for a wide range of applied and basic research questions. This service package offers the individual analysis of three plant samples by HTS to ensure a reliable detection and assembly of the viral genomes.

The end-user provided virus (e.g. as a tick homogenate) will be used to experimentally infect castor bean tick nymphs (Ixodes ricinus). The nymphs will be infected by micro-capillary inoculation into the coxal plate of the second pair of legs (transcoxal infection) using a digital microinjector system. First, 100 nymphs will be used for evaluation of the virus replication dynamics. Based on the obtained results, tick-derived virus stock will be generated from an additional 100 nymphs and provided to the end-user.

The end-user provided virus (e.g. as a tick homogenate) will be used to experimentally infect adult castor bean ticks (Ixodes ricinus). The ticks will be infected by micro-capillary inoculation into the coxal plate of the second pair of legs (transcoxal infection) using a digital microinjector system. First, 100 ticks will be used for evaluation of the virus replication dynamics. Based on the obtained results, tick-derived virus stock will be generated from an additional 100 ticks and provided to the end-user.

Virus production of viruses available on the EVAg catalogue from BMC-SAS in a single batch upon request containing 30 cryotubes containing 0.5mL.

Virus production of viruses available on the EVAg catalogue from BMC-SAS in a single batch upon request containing 30 cryotubes containing 0.5mL.

Lyophilized encapsidated RNA (ArRNA) contains a region of the MS2 phage and is used as positive control for extraction and real time RT-PCR. The ArRNA is especially developed to be spiked in nasopharyngeal, salivary, urine and stool samples. Provided with Lyophilized ready-to-use primers and probe for real time RT-PCR in a region of the MS2 phage. Positive control packaged for 100 Rxns; P&P packaged for 96 rxns in the form of 2x48 rxns Disclaimer: This product does not contain the polymerase. For RUO (Research Use Only).

Lyophilized encapsidated RNA (ArRNA) contains a region of the MS2 phage and is used as positive control for extraction and real time RT-PCR. The ArRNA is especially developed to be spiked in whole blood and serum samples. Provided with Lyophilized ready-to-use primers and probe for real time RT-PCR in a region of the MS2 phage. Positive control packaged for 100 Rxns; P&P packaged for 96 rxns in the form of 2x48 rxns Disclaimer: This product does not contain the polymerase. For RUO (Research Use Only).

Lyophilized ready-to-use primers and probe (Lyoph-P&P, 96 rxns in the form of 2x48 rxns) for real time RT-PCR in a region of the MS2 phage for internal control. Disclaimer: This product does not contain the polymerase For RUO (research use only)

We would like to invite African researchers to a training workshop on virus diagnostics. In the scope of this three-day workshop, participants from various African countries will acquire hands-on experience in the detection and characterisation of viruses using electron microscopy and Reverse Transcription Polymerase Chain Reaction (RT-PCR). This workshop offers a valuable opportunity for participants to connect and to network with each other, contributing to the prevention of virus outbreaks.

The detection of plant viruses in environmental samples, such as insects, mites, crops, weeds. obtained through high-throughput sequencing (HTS) followed by appropriate bioinformatics analyses, allows to verify the phytosanitary status of plants and is particularly useful for the identification of viruses involved in diseases with unknown etiology. The service offers the individual analysis of three samples by HTS and the downstream bioinformatic analysis, up to the identification of sequences of viral origin in each sample.

The detection of viral pathogens infecting a given plant sample, obtained through high-throughput sequencing (HTS) followed by appropriate bioinformatics analyses, allows to verify the phytosanitary status of plants and is particularly useful for the identification of viruses involved in diseases with unknown etiology or for the certification of seed and planting material. The service offers the individual analysis of three plant samples by HTS and the downstream bioinformatic analysis, up to the identification of sequences of viral origin in each sample.

Access to AMU/AFMB infrastructure for learning/training on techniques related to viral recombinant proteins

Institut Pasteur Paris - Access to BSL 3 containment facility. The access will comprise a detailed theoretical and subject to availability practical application of the biosafety and biosecurity management at Institut Pasteur. Among others, establishment and validation of suitable decontamination methods for waist, rooms and material, as well as documentation and information management will be addressed.

Kidney cells (epithelial)/ fibroblast–like, origin: Chlorocebus sabaeus (African green monkey); CRISPR/Cas9-engineered IFN-Lambda deficient phenotype

This five-day course includes two modules that can be attended separately. In the first module (Ref-SKU: 011S-02851), the basic principles of routine virus typing that can be used for hospital epidemiology and public health will be addressed by a combination of lectures, demonstration and hands-on training. Participants will visit the Viroscience clinical virology lab, where they will perform all required laboratory and analysis procedures for routine typing of viruses.

This five-day course includes two modules that can be attended separately. In the first module (Ref-SKU: 011S-02851), the basic principles of routine virus typing that can be used for hospital epidemiology and public health will be addressed by a combination of lectures, demonstration and hands-on training. Participants will visit the Viroscience clinical virology lab, where they will perform all required laboratory and analysis procedures for routine typing of viruses.

Institut Pasteur Paris - Access to BSL 3 containment facility. The access will comprise a detailed theoretical and practical application of different methods to grow lyssaviruses: strain isolation from clinical sample, virus adaptation to cell culture and Virus titration Access to BSL 3 containment facility. The access will comprise a detailed theoretical and practical application of different methods to grow lyssaviruses: strain isolation from clinical sample, virus adaptation to cell culture and Virus titration

In this 3-day course the basic principles of routine virus typing will be addressed by a combination of lectures, demonstration and hands-on training. Participants will visit the Viroscience clinical virology lab, where they will perform all required laboratory and analysis procedures for routine typing of viruses. Typing of enteroviruses will be used as an example, but the general procedures are applicable to typing of other viruses also. After this course participants should have sufficient knowledge to introduce routine typing of viruses in their own laboratory.

Access to UL infrastructure for learning/training on techniques related to virus isolation and characterization.