High-throughput sequencing has developed into a versatile standard tool in plant virology research. It provides fast and precise genomic data on the viruses being processed for a wide range of applied and basic research questions. This service package offers the individual analysis of three plant samples by HTS to ensure a reliable detection and assembly of the viral genomes.
Unit: Analysis of three individual samples, covering all steps of a workflow - from DNA/RNA extraction, state of the art library preparation, sequencing on Illumina platforms and comprehensive bioinformatics analysis.
Plasmid coding the full-length antigenome of Ebola virus isolate Makona from West African outbreak 2014. The plasmid can be used for the rescue of recombinat virus.
Plasmid coding the full-length antigenome of Ebola virus Zaire isolate Mayinga (AF086833) . The plasmid can be used for the rescue of recombinat virus.
Plasmid encoding the full-length anti-genome of Marburg virus strain Musoke under the control of the T7 polymerase for rescue experiments. The plasmid contains two genetic markers: insertion of a SacII restriction site in the GP gene (nt position 10511a to g silent mutation) and a silent mutation at nt position 9485 c toT in the GP gene resulting in the deletion of a KpnI restriction site
