EVAg

Complete cDNA of Tha genome in pSDI-HH-flash-SC vector

Expression clone for the Tha virus Nucleoprotein

Expression clone for the Tha virus Phosphoprotein

in vitro transcribed RNA for human Astro virus real time PCR

Target region of a specific Real time assay was amplified and cloned.

Plasmid for in vitro transcription that contains the 5`UTR region of an Aichi virus for 5`UTR-Leader region PCR assays published by Drexler et al, Emerg Infect Dis. 2011

Human Coronavirus 229E plasmid for in vitro transcription.

in vitro transcribed RNA for Human coronavirus 229E real time PCR

In vitro transcribed RNA for Wuhan coronavirus (2019-nCoV) real time (RT-)PCR targeting region of the E gene . Protocol and preliminary evaluation for this assays (Corman/Drosten 2020), as of Jan 13, 2020, available from: https://www.who.int/docs/default-source/coronaviruse/protocol-v2-1.pdf?sfvrsn=a9ef618c_2 Ref: https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2020.25.3.2000045

In vitro transcribed RNA for Wuhan coronavirus (2019-nCoV) real time (RT-)PCR targeting region of the RdRP gene . Protocol and preliminary evaluation for this assays (Corman/Drosten 2020), as of Jan 13, 2020, available from: https://www.who.int/docs/default-source/coronaviruse/protocol-v2-1.pdf?sfvrsn=a9ef618c_2 Ref: https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2020.25.3.2000045

RNA extracted from infected cell culture supernatant containing SARS-CoV-2 (strain hCoV-19/Slovenia/11454/21 (lineage B 1.1.7, exUK))

RNA extracted from infected cell culture supernatant containing SARS-CoV-2 (strain hCoV-19/Aruba_11401/2021, California variant, nextstrain clade CAL.20C, lineage B.1.429).

RNA extracted from infected cell culture supernatant containing SARS-CoV-2 strain hCoV-19/Netherlands/Gelderland_20949/2021, N501T (S), nextstrain clade 20D, lineage C.2.

RNA extracted from infected cell culture supernatant containing SARS-CoV-2 strain hCoV-19/Netherlands/NoordBrabant_10706/2021, Brazilian variant P.2, nextstrain clade 20B, lineage B.1.1.28 P.2.

RNA extracted from infected cell culture supernatant containing SARS-CoV-2 strain hCoV-19/Netherlands/NoordHolland_10915/2021, Brazilian variant P.1, nextstrain clade 20J, lineage B.1.1.28 P.1.

Double-stranded DNA copy of a coxsackievirus A17 genome without the capsid-encoding region, cloned into a plasmid backbone downstream a T7 promoter. After in vitro transcription, the corresponding RNA can be transfected into cells to give rise to a replicon that is self-replicative and can express the non-structural virus proteins but is unable to produce infectious particles. The plasmid can be amplified in E. coli.

Infectious clone: double-stranded DNA copy of the genome of Coxsackievirus A17 strain 67591 downstream a T7 promoter in a plasmid backbone. In vitro transcribed RNA produces the virus upon transfection of permissive cells. The plasmid can be amplified in E. coli.

RNA extracted from infected cell culture supernatant containing SARS-CoV-2 strain hCoV-19/Netherlands/NoordHolland_10159/2021, South African variant, nextstrain clade 20H, lineage B.1.351).

Grapevine pinot gris virus, isolate SK30. Complete genome sequence cloned in pCass2 vector.

Reverse genetics-based life cycle modelling system that allows modelling of Ebola virus genome replication and transcription, viral protein synthesis, virus assembly, budding, and entry into target cells under BSL1 conditions.

Reverse genetics-based life cycle modelling system that allows modelling of Ebola virus genome replication and transcription, viral protein synthesis, virus assembly, budding, and entry into target cells under BSL1 conditions.

Reverse genetics-based life cycle modelling system that allows modelling of Ebola virus genome replication and transcription, viral protein synthesis, virus assembly, budding, and entry into target cells under BSL1 conditions.

Reverse genetics-based life cycle modelling system that allows modelling of Ebola virus genome replication and transcription, viral protein synthesis, virus assembly, budding, and Sudan virus entry into target cells under BSL1 conditions.

Reverse genetics-based life cycle modelling system that allows modelling of Ebola virus genome replication and transcription, viral protein synthesis, virus assembly, budding, and Sudan virus entry into target cells under BSL1 conditions.

Reverse genetics-based life cycle modelling system (8 plasmids) that allows modelling of Ebola virus genome replication and transcription, viral protein synthesis, virus assembly, budding, and Reston virus entry into target cells under BSL1 conditions.