EVAg

Infectious cell culture supernatant containing SARS-CoV-2, JN.1

Virus was produced from plasmid-based DNA segments of a common Wuhan strain. The S-gene was site-specifically replaced with a cloned BA.1-based S-gene sequence.

Full length of SARS-CoV-2 N protein with C-terminal 6xHis tag in 0.1% SDS/PBS. IMAC purified using HisTrap HP column. NCBI Reference Sequence: YP_009724397.2 GenBank: QHD43423.2

Recombinant non-structural protein Nsp1 (leader protein, aa 2- 180) of SARS-CoV-2 (Wuhan). The protein was expressed in E.coli BL21 and Streptactin-purified.

Receptor-binding domain aa 319 - 519 of SARS-CoV-2 (Wuhan), expressed in Expi293 cells and Streptactin-purified. The protein is equipped with a SpyTag (AHIVMVDAYKPTK) at the C-terminus.

Infectious cellculture supernatant of human 2019-nCoV

In vitro transcribed RNA for Wuhan coronavirus (2019-nCoV) real time (RT-)PCR targeting region of the E gene . Protocol and preliminary evaluation for this assays (Corman/Drosten 2020), as of Jan 13, 2020, available from: https://www.who.int/docs/default-source/coronaviruse/protocol-v2-1.pdf?sfvrsn=a9ef618c_2 Ref: https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2020.25.3.2000045

In vitro transcribed RNA for Wuhan coronavirus (2019-nCoV) real time (RT-)PCR targeting region of the RdRP gene . Protocol and preliminary evaluation for this assays (Corman/Drosten 2020), as of Jan 13, 2020, available from: https://www.who.int/docs/default-source/coronaviruse/protocol-v2-1.pdf?sfvrsn=a9ef618c_2 Ref: https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2020.25.3.2000045

Infectious cell culture supernatant containing SARS-CoV-2 (strain hCoV-19/Slovenia/11454/21 (lineage B 1.1.7, exUK)). This strain is preserved in viral storage medium -80°C.

The in vitro transcribed RNA is the control for the first-line screening assay termed E gene assay. The SARS-CoV full virus RNA is required to control the two confirmatory assays. Dislaimer: Import permit maybe be needed in your country for the SARS-CoV full virus RNA. If yes, please indicate so in your online enquiry.