EVAg

Infectious cell culture supernatant containing SARS-CoV-2, JN.1

Virus was produced from plasmid-based DNA segments of a common Wuhan strain. The S-gene was site-specifically replaced with a cloned BA.1-based S-gene sequence.

Full length of SARS-CoV-2 N protein with C-terminal 6xHis tag in 0.1% SDS/PBS. IMAC purified using HisTrap HP column. NCBI Reference Sequence: YP_009724397.2 GenBank: QHD43423.2

Recombinant non-structural protein Nsp1 (leader protein, aa 2- 180) of SARS-CoV-2 (Wuhan). The protein was expressed in E.coli BL21 and Streptactin-purified.

Receptor-binding domain aa 319 - 519 of SARS-CoV-2 (Wuhan), expressed in Expi293 cells and Streptactin-purified. The protein is equipped with a SpyTag (AHIVMVDAYKPTK) at the C-terminus.

SARS-CoV-2 S1 protein The Protein was expressed in Expi293F cells and purified using HisTrap HP column followed by size exclusion chromatography. It is provided in PBS, pH7.4.

Infectious cellculture supernatant of human 2019-nCoV

In vitro transcribed RNA for Wuhan coronavirus (2019-nCoV) real time (RT-)PCR targeting region of the E gene . Protocol and preliminary evaluation for this assays (Corman/Drosten 2020), as of Jan 13, 2020, available from: https://www.who.int/docs/default-source/coronaviruse/protocol-v2-1.pdf?sfvrsn=a9ef618c_2 Ref: https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2020.25.3.2000045

In vitro transcribed RNA for Wuhan coronavirus (2019-nCoV) real time (RT-)PCR targeting region of the RdRP gene . Protocol and preliminary evaluation for this assays (Corman/Drosten 2020), as of Jan 13, 2020, available from: https://www.who.int/docs/default-source/coronaviruse/protocol-v2-1.pdf?sfvrsn=a9ef618c_2 Ref: https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2020.25.3.2000045

Infectious cell culture supernatant containing SARS-CoV-2 (strain hCoV-19/Slovenia/11454/21 (lineage B 1.1.7, exUK)). This strain is preserved in viral storage medium -80°C.

The in vitro transcribed RNA is the control for the first-line screening assay termed E gene assay. The SARS-CoV full virus RNA is required to control the two confirmatory assays. Dislaimer: Import permit maybe be needed in your country for the SARS-CoV full virus RNA. If yes, please indicate so in your online enquiry.