EVAg

In-house slides to detect Orthopoxvirus antibodies by Immunofluorescence assay. The slides are fixated and preserved at -20°C.

IF-slides to detect anti-nCoV antibodies by Immunofluorescence assay. The slides were prepared by infecting Vero cells with the first Swedish SARS-CoV-2 isolate (SARS-CoV-2/01/human/2020/SWE). The virus is fully sequenced (Genebank accession: MT093571). The slides are fixated in acetone, air-dried and preserved at -80°C. IF-analysis tested positive with SARS-CoV-2 infected human sera.

In-house IF-slides to detect anti-CCHFV antibodies by Immunofluorecence assay. The slides were prepared by infecting Vero cells with the CCHFV strain, Nigerian IbAr10200. The slides are fixated in acetone, air-dried and preserved at -80°C. The slide has been tested positive with mouse monoclonal anti-CCHFV antibodies against N, Gc and Gn.

In-house slides to detect anti-EBOV antibodies by Immunofluorecence assay. The slides are fixated and preserved at -80°C. The slide has been tested positive with three different anti-EBOV antibodies; anti-Gp, anti-VP40, and anti-NP antibodies.

In this 3-day course the basic principles of the protein microarray will be addressed by a combination of lectures, demonstration and hands-on training. The protein microarray technique can be used for HA antibody profiling for group level assessment of exposures to influenza viruses in small sample volumes from humans and animals (birds, horses, pigs, bats, carnivores a.o.). The training encompasses wet lab training but also the analysis and interpretation of assay results. Special attention will be given to specific issues of Influenza and flavivirus cross reactivity.

24 pad nitrocellulose glass slides with a set of 33 HA1 proteins printed on each pad. The slides can be used for HA antibody profiling for group level assessment of exposures to influenza viruses in small sample volumes from humans and animals (birds, horses, pigs, bats, carnivores a.o.) in very small volumes of serum (10 μL). Each slides can be used for 24 sera. Scanning and analysis services are included.