EVAg

The lyophilized, non infectious, encapsidated RNA (Armored RNA) contains the sequence of the NS5 region from Usutu virus and can be used as positive control for extraction and real time RT-PCR using specific Primers and probe ref: 001K-06005. Extraction is mandatory before use in real time RT-PCR. Packaged for 100 Rxns, for RUO (Research Use Only). SOPs in the attached pdf.

Freeze-dried human plasma spiked with heat inactivated and diluted Usutu virus culture supernatant, (non infectious)

Freeze-dried Usutu virus strain UVE/USUV/1959/ZA/SAAR-1776 , South Africa, 1959, complete ORF sequence.

Freeze-dried Usutu virus, strain UVE/USUV/2023/FR/CNR_Cx36-2, France , 2023, complete genome sequence.

Lyophilized ready-to-use primers and probe (Lyoph-P&P, 96 rxns in the form of 24x4 rxns) for real time RT-PCR of Usutu virus (Taqman) SOPs in the attached pdf. Disclaimer: This product does not contain the polymerase. For RUO (research use only).

In vitro transcribed RNA (positive control) for a Usutu virus real time PCR. (Ref: J Virol Methods. 2014 Mar;197:51-4. doi: 10.1016/j.jviromet.2013.08.039. Epub 2013 Sep 13, "Development of a Usutu virus specific real-time reverse transcription PCR assay based on sequenced strains from Africa and Europe." Nikolay B1, Weidmann M2, Dupressoir A3, Faye O4, Boye CS5, Diallo M6, Sall AA7.)

Live, frozen Usutu Virus in tissue culture supernatant. Strain 3345, Isolate Arb276. This Usutu virus is preserved under viral storage medium at -80oC. The virus product tested negative for Mycoplasmae.

Infectious cell culture supernatant. This Usutu virus[Slovenia,Ko 208/18] is freeze dried. Virus is mycoplasma free. To confirm its identity the virus has been fully sequenced.

Usutu virus, strain IP/USUV/09/03/2009

infectious cell culture supernatant. This Usutu virus[1477] is preserved under Viral Storage Medium -80C.Tests for the presence of mycoplasmae were not performed.

Usutu virus

Usutu virus

Infectious cell culture lysate. This Usutu virus[Vienna 2001-939/01] is preserved under Viral Storage Medium -80C. Tests for the presence of mycoplasmae were positive.

Viral RNA extracted from infected cell culture lysate

cDNA from Usutu Virus, isolate Arb276_3345. Specific primer Usutu Jost For.

RNA from Usutu Virus, isolate Arb276_3345, in HPLC water

Expression plasmids for the production of flaviviruses NS5 full length (MTase and RdRp domains). Synthetic gene optimized for E. coli. Coding sequence fused at its 5' with a cleavage 6His tag coding sequence