Aeonium ringspot virus, RNA1 and RNA2 full-length cDNAs are cloned in plasmid pUC18, under the control of a T7 promoter, allowing for transcription of infectious RNAs.
Unit: 2 micrograms of each plasmid DNA, allowing for the inoculation of six host plants (Nicotiana benthamiana) after T7-driven transcription
The 3' terminal 217 nts of Aeonium ringspot virus RNA1 and RNA2 (excluding the poly(A) tail) are cloned in pGEM-T Easy allowing for transcription of a riboprobe to detect both genomic RNAs.
Unit: 2 micrograms of plasmid DNA, allowing for the synthesis of a probe after T7-driven transcription
Neuraminidase subtype N2 (amino acids 30 - 461) from Influenza A virus subtype H9N2 (A/Bat/Egypt/381OP/2017). The protein is equipped with a c-terminal FLAG-tag and was expressed in Expi293 cells. Purification was performed using FLAG-M2 affinity chromatography followed by dialysis against 1X PBS pH 7.4.
