Product Description
Ref-SKU:
008v-EVA1491 TBEV isolate from our collection (IVSAS). This Tick-borne encephalitis virus[114] is preserved under Viral Storage Medium -80C.Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been partly sequenced.
Product Risk Group:
ICTV Taxonomy:
Virus name:
Isolate:
Storage conditions:
Viral Storage Medium -80C
Sequencing:
Complete genome
Infectivity:
Infectivity tested and quantified
Mycoplasmic content:
Mycoplasma free
GMO:
No
Virus host type:
Infectivity Test:
Infectivity was proven by plaque titration. Infectious titre was determined to be 2x 10^7 PFU/ml.
Production cell line:
Genbank reference:
Virus Cultivability:
Passage:
Passaged 9 times on suckling mice on 29th May 1991 and stored freeze-dried. New stock prepared by one passage on Vero E6 cells on 23rd August 2013.
Identification technique:
RT-PCR followed by sequencing of the amplified product.
Technical recommendation:
We recommend to grow the virus on VeroE6 cells cultivated in MEM Eagle with Earle"s BSS (BioWhittaker, 12-136F). Following additives should be added to 500 ml of medium: 5ml Nonesential amino acids (Biochrom AG, K0293), 5 ml Sodium Pyruvate 100mM, (Biochrom AG, L0473), 5 ml L-Alanyl-L-glutamine 200mM (Biochrom AG, K0302), 5 ml Penicilin/ Streptomycin/ Amphotericin (BioWhittaker 17-745E), 25 ml FBS heat inact.s.American (Invitrogen, 10108-165). Multiplicity of infection (MOI) of 0,1 can be used for the infection and the infectious medium can be harvested 96 hours after infection.
Shipping conditions:
IATA Classification:
Information about the collection of the virus
Recombinant product:
No
Biological material origin:
Natural origin
Collection date: BEFORE
Tuesday, 1 January, 1980
Country of collection:
Suspected epidemiological origin :
Isolation host: