Tick-borne encephalitis virus (TBEV), genotype TBEV-Eu, divergent strain Salland (the Netherlands) rescued and cultured on BHK-21 cells
Product Description
Ref-SKU:
014V-05209 Tick-borne encephalitis virus (TBEV), subtype TBEV-Eu, divergent strain Salland (the Netherlands) rescued and cultured on BHK-21 cells as described in Hoornweg et al., Sci Rep. 2023 Feb 18;13(1):2872. doi: 10.1038/s41598-023-29075-0 (https://www.nature.com/articles/s41598-023-29075-0).
This strain is preserved in viral storage medium -80°C.
Product Risk Group:
ICTV Taxonomy:
Virus name:
Strain:
Genotype:
Storage conditions:
Viral Storage Medium -80C
Sequencing:
Complete genome
Infectivity:
Infectivity tested and quantified
Mycoplasmic content:
Not tested
GMO:
Yes
Biosafety restrictions:
BSL3
Virus host type:
Infectivity Test:
PRNT
Production cell line:
Virus Cultivability:
Passage:
2
Technical recommendation:
0.01 MOI on BHK-21 cells 3dpi
Shipping conditions:
IATA Classification:
Information about the collection of the virus
Recombinant product:
This product is a recombinant product
Biological material origin:
Synthetic origin & Natural origin
Multiple reference sequences were used from the public databases for the design of this product
Natural part identification: Almost complete genome of TBEV Salland ex-Ix. ricinus with 7 nts at the 5' end and 2 nts at the 3'end left unsolved in comparison to full length TBEV genomes.
Natural part identification:
Almost complete genome of TBEV Salland ex-Ix. ricinus with 7 nts at the 5' end and 2 nts at the 3'end left unsolved in comparison to full length TBEV genomes.
Genbank reference:
Collection date:
Friday, 4 September, 2015
Country of collection:
Suspected epidemiological origin :
Isolation host:
Isolation technique:
virus rescue using amplicons based on genome sequence
Isolation conditions:
see Hoornweg et al
Synthetic part identification: 7 nts at 5' end and 2 nts at 3'end. The genome was completed by adding 5′-AGATTTT-3′ (5′-UTR; conserved in 97% of tick-borne flavivirus genomes) and 5′-CT-3′ (3′-UTR; conserved in 100% of tick-borne flavivirus genomes) to the extreme ends of the genome.
Synthetic part identification:
7 nts at 5' end and 2 nts at 3'end. The genome was completed by adding 5′-AGATTTT-3′ (5′-UTR; conserved in 97% of tick-borne flavivirus genomes) and 5′-CT-3′ (3′-UTR; conserved in 100% of tick-borne flavivirus genomes) to the extreme ends of the genome.
Genbank reference:
Modifications from the reference sequence(s):
The sequence(s) of this synthetic product has NOT been modified from the sequence listing provided as Genbank references