Product Description
Ref-SKU:
008V-03724 Dobrava-Belgrade virus strain SK/Aa. This Dobrava-Belgrade virus[Slovakia/Aa] is freeze-dried.Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been partly sequenced.
Product Risk Group:
ICTV Taxonomy:
Virus name:
Strain:
Genotype:
Storage conditions:
Freeze Dried
Sequencing:
Partial sequence
Infectivity:
Infectivity tested and quantified
Mycoplasmic content:
Mycoplasma free
GMO:
No
Virus host type:
Infectivity Test:
Focus assay
Production cell line:
Virus Cultivability:
Technical recommendation:
We recommend to grow the virus on VeroE6 cells cultivated in MEM Eagle with Earle"s BSS (BioWhittaker, 12-136F). Following additives should be added to 500 ml of medium: 5ml Nonesential amino acids (Biochrom AG, K0293), 5 ml Sodium Pyruvate 100mM, (Biochrom AG, L0473), 5 ml L-Alanyl-L-glutamine 200mM (Biochrom AG, K0302), 5 ml Penicilin/ Streptomycin/ Amphotericin (BioWhittaker 17-745E), 25 ml FBS heat inact.s.American (Invitrogen, 10108-165). Multiplicity of infection (MOI) of 0,001 can be used for the infection and the infectious medium can be harvested 7 days after infection.
Shipping conditions:
IATA Classification: