EVAg

Many regularly cultivated field crops are affected by viral pathogens, especially if they are grown on a large scale, which usually results in significant yield reductions. As a first step, it is important to obtain an overview of which viruses are involved in order to counter this problem. To obtain a representative overview, it is necessary to investigate a significant number of samples.

Many regularly cultivated field crops are affected by viral pathogens, especially if they are grown on a large scale, which usually results in significant yield reductions. As a first step, it is important to obtain an overview of which viruses are involved in order to counter this problem. To obtain a representative overview, it is necessary to investigate a significant number of samples.

In this 3-day course the basic principles of the protein microarray will be addressed by a combination of lectures, demonstration and hands-on training. The protein microarray technique can be used for HA antibody profiling for group level assessment of exposures to influenza viruses in small sample volumes from humans and animals (birds, horses, pigs, bats, carnivores a.o.). The training encompasses wet lab training but also the analysis and interpretation of assay results. Special attention will be given to specific issues of Influenza and flavivirus cross reactivity.

24 pad nitrocellulose glass slides with a set of 33 HA1 proteins printed on each pad. The slides can be used for HA antibody profiling for group level assessment of exposures to influenza viruses in small sample volumes from humans and animals (birds, horses, pigs, bats, carnivores a.o.) in very small volumes of serum (10 μL). Each slides can be used for 24 sera. Scanning and analysis services are included.