EVAg

Methods of diagnostic of influenza and ARVI. Algorithms of investigation. Organisation of a PCR lab. Collection and transport of samples. Biosafety in a PCR lab. Isolation of nucleic acids from nasal swabs (RNeasy MiniKit). Conservation of isolated nucleic acids. Reverse-transcription. One-step and two-step PCR.Criterions of the quality control of biological material (RNP). Real-time amplificators. Design of operational software. AmplySense influenza virus A/B and ARVI-screen. Treatment of wastes after PCR-diagnostics. Analysis of results of PCR. Desing of databases. Principles of sequencing and phylogenetic analysis. Examples of analysis of phylogenetic trees.