Product Description
Ref-SKU:
029N-05165 Pothos latent virus full-length cDNA is cloned in plasmid pUC18, under the control of a T7 promoter, allowing for transcription of infectious RNA
Product Risk Group:
Information on the related virus
ICTV Taxonomy:
Virus name:
Cloned Product: Yes
Can it be used to produce GMO:
No
Region encompassed in this Product:
complete genome
Plasmid:
Plasmid selection:
TAG:
Sequencing:
Fully sequenced
Sequence checked:
Yes
Mutations:
Contain mutations (no frameshift, no unexpected STOP codon)
Observed mutations:
in the plasmid, the 5' nucleotide of the PoLV sequence is G instead of A. This allows efficient in vitro transcription
Genbank reference:
Nucleic Acid preparation technique:
Plasmid amplification in Escherichia coli DH5alpha. DNA preparation obtained by alkaline lysis, phenol extraction and PEG precipitation
Storage conditions:
Nuclease-Free Water -20C
Shipping conditions:
IATA Classification:
Virus host type:
Information about the origin of the product
Recombinant product:
This product is a recombinant product
Biological material origin:
Synthetic origin & Natural origin
Synthetic part identification: Cloned in pUC18. The 5' terminal A has been replaced by G to allow efficient T7 driven transcription
Synthetic part identification:
Cloned in pUC18. The 5' terminal A has been replaced by G to allow efficient T7 driven transcription
Modifications from the reference sequence(s):
The sequence(s) of this synthetic product has been modified from the sequence listing provided as Genbank references
Description of modification(s) made from the reference sequence(s):
The 5' terminal A has been replaced by G to allow efficient T7 driven transcription