HIV-1 pNL-BX_prM46I-I47L-I50V-V82A-I84V
nucleic-acid

HIV-1 pNL-BX_prM46I-I47L-I50V-V82A-I84V

ANTIVIRAL RESISTANT STRAIN

EVAg
Produced by
: UNIBAS
Shipping From
: Basel - CH

Product Description

Ref-SKU:
006N-02602
Protease mutant with the indicated discrete mutations relevant for drug resistance to protease inhibitors
Product Risk Group: 
RG2

Information on the related virus

ICTV Taxonomy: 
Riboviria / Pararnavirae / Artverviricota / Revtraviricetes / Ortervirales / Retroviridae / Orthoretrovirinae / Lentivirus / Human immunodeficiency virus 1
Virus name: 
Human immunodeficiency virus 1
Subspecies: 
Group M
Strain: 
Clade B


Unit definition: 
10 ng purified plasmid DNA

In stock

675,00 €
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Cloned Product: Yes
Special feature: 
Antiviral resistant strain
Can it be used to produce GMO: 
No
Biosafety restrictions: 
May reconstitute infectious virus after transfection into suitable human cells
Region encompassed in this Product: 
full length viral clone
Plasmid: 
pUC 18 (Pvu II)
Plasmid selection: 
Ampicillin
TAG: 
No Tag
Sequencing: 
Fully sequenced
Sequence checked: 
Yes
Mutations: 
Contain mutations (no frameshift, no unexpected STOP codon)
Observed mutations: 
The virus genome is derived from pNL-NF (pNL4-3 with shortened cellular flank) (doi.org/10.1016/j.jcv.2006.05.001). mutations as indicated in the name; backbone is NL-BX. A unique BamHI site was introduced 5’ of protease (ggagcc2207ggaTcc; A->S in Pol; E->D in Gag) and an XmaI site 3’ of protease (ccagga2589ccCggG). The original BamHI site as position 8465 was removed (GGATCC->GGATCA change silent in Env; L->S in Rev). Other mutations as indicated in the product name.
Storage conditions: 
TE Buffer -20C
Shipping conditions: 
Room Temperature
IATA Classification: 
NON DANGEROUS GOODS
Virus host type: 
Human virus
Note: 

To identify the resistance(s) to antiretroviral drugs caused by the mutations present in this viral clone, use e.g. the „mutations list analysis“ function of the public HIV-Grade site at:

www.hiv-grade.de/grade_new/

Information about the origin of the product

Recombinant product: 
This product is a recombinant product
Biological material origin: 
Synthetic origin & Natural origin

Natural part identification: 
NL4-3

Genbank reference: 
AF324493
Collection date: BEFORE 
Wednesday, 1 September, 2010
Country of collection: 
United States of America (the)

Synthetic part identification: 
combination of mutations in protease positions: 46Ile, 47Leu, 50Val, 82Ala, 84Val

Modifications from the reference sequence(s): 
The sequence(s) of this synthetic product has been modified from the sequence listing provided as Genbank references
Description of modification(s) made from the reference sequence(s): 
PCR-based mutagenesis; carries the indicated mutation(s) in the target gene as shown in the product name