Product Description
Ref-SKU:
006N-03076 Plasmid contains the entire genome of HIV-1 with specific mutations in the protease gene, which can confer drug resistance to specific HIV-1 PR inhibitors. The respective mutations have been reported in viruses from HIV-infected individuals on PR inhibitors with virologic therapy failure.
Product Risk Group:
Information on the related virus
ICTV Taxonomy:
Virus name:
Subspecies:
Strain:
Isolate:
Cloned Product: Yes
Special feature:
Can it be used to produce GMO:
No
Biosafety restrictions:
Plasmid contains the entire genome of HIV-1 with specific mutations in the protease gene, which can confer drug resistance to specific protease inhibitors. Might produce infectious virus after transfection into suitable human cells.
Region encompassed in this Product:
entire HIV-1 genome
Plasmid:
Plasmid selection:
TAG:
Sequencing:
Fully sequenced
Sequence checked:
Yes
Mutations:
Contain mutations (no frameshift, no unexpected STOP codon)
Observed mutations:
The virus genome is derived from pNL-NF (pNL4-3 with shortened cellular flank) (doi.org/10.1016/j.jcv.2006.05.001).
mutations as indicated in the name; backbone is NL-BX.
A unique BamHI site was introduced 5’ of protease (ggagcc2207ggaTcc; A->S in Pol; E->D in Gag) and an XmaI site 3’ of protease (ccagga2589ccCggG).
The original BamHI site as position 8465 was removed (GGATCC->GGATCA change silent in Env; L->S in Rev).
Other mutations as indicated in the product name.
Storage conditions:
TE Buffer -20C
Shipping conditions:
IATA Classification:
Virus host type:
Note:
To identify the resistance(s) to antiretroviral drugs caused by the mutations present in this viral clone, use e.g. the „mutations list analysis“ function of the public HIV-Grade site at:
Information about the origin of the product
Recombinant product:
This product is a recombinant product
Biological material origin:
Synthetic origin & Natural origin
Natural part identification: NL4-3
Natural part identification:
NL4-3
Genbank reference:
Collection date: BEFORE
Wednesday, 1 September, 2010
Country of collection:
Synthetic part identification: Point mutations in the protease gene, position L24I
Synthetic part identification:
Point mutations in the protease gene, position L24I
Modifications from the reference sequence(s):
The sequence(s) of this synthetic product has been modified from the sequence listing provided as Genbank references
Description of modification(s) made from the reference sequence(s):
PCR-based mutagenesis; carries the indicated mutation(s) in the target gene as shown in the product name