Originator
: Agricultural Biotechnology Center, Godollo, Hungary
Produced by
: CNR Shipping From
: Roma - IT
Product Description
Ref-SKU:
029N-05160 Cymbidium ringspot virus DI-3 full-length cDNA cloned in plasmid pUC18, under the control of a T7 promoter, allowing for transcription of biologically active RNA
Product Risk Group:
Information on the related virus
ICTV Taxonomy:
Virus name:
Cloned Product: Yes
Can it be used to produce GMO:
No
Region encompassed in this Product:
complete DI-3 sequence
Plasmid:
Plasmid selection:
TAG:
Sequencing:
Fully sequenced
Sequence checked:
Yes
Mutations:
Contain mutations (no frameshift, no unexpected STOP codon)
Observed mutations:
in the plasmid, the 5' nucleotide of the DI-3 sequence is G instead of A. This allows efficient in vitro transcription. The clone contains two engineered restriction sites, HpaI and EcoRV, which do not affect DI RNA replication
Nucleic Acid preparation technique:
Plasmid amplification in Escherichia coli DH5alpha. DNA preparation obtained by Euroclone Spinnaker Plasmid DNA miniprep kit.
Storage conditions:
Nuclease-Free Water -20C
Shipping conditions:
IATA Classification:
Virus host type:
Information about the origin of the product
Recombinant product:
This product is a recombinant product
Biological material origin:
Synthetic origin & Natural origin
Synthetic part identification: Cloned in pUC18. HpaI and EcoRV restriction sites introduced by site-directed mutagenesis
Synthetic part identification:
Cloned in pUC18. HpaI and EcoRV restriction sites introduced by site-directed mutagenesis
Modifications from the reference sequence(s):
The sequence(s) of this synthetic product has been modified from the sequence listing provided as Genbank references
Description of modification(s) made from the reference sequence(s):
HpaI and EcoRV restriction sites introduced by site-directed mutagenesis