- Tagged as
- - Derived product
- - Nucleic Acid
- - Cloned nucleic acid
Produced by
: IP Shipping From
: Paris - FR
Product Description
Ref-SKU:
017N-04351 Complete cDNA of Th4M genome cloned in pSDI-HH-flash-SC vector
Product Risk Group:
Information on the related virus
ICTV Taxonomy:
Virus name:
Isolate:
Genotype:
Cloned Product: Yes
Can it be used to produce GMO:
Yes
Region encompassed in this Product:
cDNA encoding the complete genome of Th4M recombinant virus
Plasmid:
Plasmid selection:
TAG:
Sequencing:
Fully sequenced
Sequence checked:
Yes
Mutations:
Contain mutations (no frameshift, no unexpected STOP codon)
Observed mutations:
Modification in the non coding region between the M and the G gene ; deletion of 10 nucleotides and insertion of a PacI restriction site to be able to insert reporter gene (see the sequence below).
Mutations are introduced in the P gene to obtain 2 amino acid modifications (W in position 265 of the P protein replaced by G ; M in position 287 of the P protein replaced by V)
Genbank reference:
.gb file of the construct:
Nucleic Acid preparation technique:
Maxipreparation of plasmid
Storage conditions:
Nuclease-Free Water -20C
Shipping conditions:
IATA Classification:
Virus host type:
Note:
The pSDI-HH-flash-SC vector was a gisft from Pr. K.K. Conzelmann.
Information about the origin of the product
Recombinant product:
This product is a recombinant product
Biological material origin:
Synthetic origin & Natural origin
Natural part identification: Complete cDNA of Tha virus mutated on four positions of the M gene (R in position 77 by K (R77K); D100A, A104S and M110L)
Natural part identification:
Complete cDNA of Tha virus mutated on four positions of the M gene (R in position 77 by K (R77K); D100A, A104S and M110L)
Genbank reference:
Collection date:
Thursday, 1 January, 1987
Country of collection:
Synthetic part identification: pSDI-HH-flash-SC
Synthetic part identification:
pSDI-HH-flash-SC
Genbank reference:
Modifications from the reference sequence(s):
The sequence(s) of this synthetic product has NOT been modified from the sequence listing provided as Genbank references