Product Description
Ref-SKU:
026S-03568 Cell line: S.hisp AEC.B - Organism: Sigmodon hispidus - Organ: Lung - Cell type: Airway Epithelium - Genotyped: Yes - Passage No: 6
Product Risk Group:
Information on the related virus
ICTV Taxonomy:
Virus name:
Can it produce GMO:
No
Technical recommendation:
Growth Properties: Adherent/ culture at 37°C and 5% CO2- Culture Medium: DMEM (Dulbecco's Modified Eagles Medium) (PAA, Cölbe, Germany) with 4.5 g/L Glucose (PAA) supplemented with 10% Foetal Bovine Serum (PAA), 1% Penicillin/Streptomycin 100 x concentrate (Penicillin 10000 U/mL, Streptomycin 10 mg/ mL) (PAA), 1% L-Glutamine 200 mM, 1% Sodium Pyruvate 100 mM (PAA), 1% MEM nonessential amino acids (NEAA) 100 x concentrate (PAA)Subculture routine: Detachment with an EDTA/trypsin mixture (PAA)- Split Ratio: Split 1:20 twice a week