Real time detection kit for Zika virus targeting the matrix gene. The kit contains positive control, primers and probe for 50 reactions. Real time primers and probe were published by Lanciotti RS, Kosoy OL, Laven JJ, Velez JO, Lambert AJ, Johnson AJ, et al. Genetic and serologic properties of Zika virus associated with an epidemic, Yap State, Micronesia, 2007. Emerg Infect Dis. 2008 Aug;14(8):1232-9.”