EVAg

Infectious Subgenomic Amplicons Reverse Genetic system for Zika virus strain Dak84 (derived from genbank sequence KU955592). It consists of three non infectious overlapping DNA fragments (cloned into pUC57 vector). These products enable to obtain infectious RNA virus after amplification by PCR, and transfection into cells,

Infectious Subgenomic Amplicons Reverse genetic system for Zika virus strain H/PF/2013. It consists of three non infectious overlapping DNA fragments (cloned into pUC57 vector). These products enable to obtain infectious RNA virus after amplification by PCR, and transfection into cells,

Subgenomic Amplicons for Zika virus strain H/PF/2013. It consists of three non infectious overlapping DNA fragments (amplicons). These products enable to obtain infectious RNA virus after transfection into cells.

Subgenomic Amplicons for Zika virus strain Dak84 (derived from genbank sequence KU955592). It consists of three non infectious overlapping DNA fragments (amplicons). These products enable to obtain infectious RNA virus after transfection into cells

A specific RT-PCR was performed on KJ776791. The product was cloned into a vector, sequenced and in vitro transcribed. In vitro transcript: Vector Sequence: Position 1-36, 727-816 Insert Sequence: Position 37-726

A specific genome region was amplified by PCR, cloned into a vector and in vitro transcribed. In vitro transcript: Vector Sequence: 1-36, 632-721 Insert Sequence: 37-631

A specific RT-PCR was performed on KJ776791. The product was cloned into a vector, sequenced an in vitro transcribed. In vitro transcript: Vector Sequence: 1-36, 638-727 Insert Sequence: 37-637

A specific RT-PCR was performed on KJ776791. The product was cloned into a vector, sequenced an in vitro transcribed. In vitro transcript: Vector Sequence: 1-36, 674-763 Insert Sequence: 37-673

A specific RT-PCR was performed on KJ776791. The product was cloned into a vector, sequenced an in vitro transcribed. In vitro transcript: Vector Sequence: 1-36, 634-723 Insert Sequence: 37-633

In vitro transcribed RNA containing priming sites for different zika virus real time PCRs.

In vitro transcribed RNA that contains priming sites for different Zika virus rt-PCRs, encapsulated within a protective protein coat.

RNA extracted from infected cell culture supernatant

Lama serum obtained by immunization with the recombinant ZIKV DIIIE + positive control (recombinant DIIIE)

Pure recombinant protein for enzymatic assay and structural studies. MTase domain including amino acid 1 to 264 of ZIKV NS5, with a N-terminal 6-His tag.

Expression plasmid for the production of the Nterminal - Domain of ZIKV NS5 (methyltransferase domain). Synthetic gene optimized for E. coli. Coding sequence fused at its 5' with a 6His tag coding sequence

Expression plasmid for the production of the Nterminal - Domain of ZIKV NS5 (methyltransferase domain, amino acids 4-278). Synthetic gene optimized for E. coli. Coding sequence fused at its 5' with a 6His tag coding sequence

Pure recombinant protein for enzymatic assays. MTase domain (amino acid 4 - 278 of NS5, fused to a N-terminal 6-His tag )

Set of primers and probes to detect viral RNA of Zika virus on diagnostic level. Suitable with the EVD-labnet / EVAg EQA of molecular diagnostics on Chikungunya, Dengue, West Nile, Yellow fever and Zika virus 2019.

Real time detection kit for Zika virus targeting the envelope gene. The kit contains positive control, primers and probe for 50 reactions. Real time primers and probe were published by “Lanciotti RS, Kosoy OL, Laven JJ, Velez JO, Lambert AJ, Johnson AJ, et al. Genetic and serologic properties of Zika virus associated with an epidemic, Yap State, Micronesia, 2007. Emerg Infect Dis. 2008 Aug;14(8):1232-9.”

rabbit polyclonal antiserum against the pre-membrane protein (prM) of ZIKV

rabbit polyclonal antiserum against the envelop protein (prM) of ZIKV

Real time detection kit for Zika virus targeting the envelope gene. The kit contains positive control, primers and probe for 50 reactions. Real time primers and probe were published by “Corman VM, Rasche A, et al. Clinical comparison, standardization and optimization of Zika virus molecular detection Submitted]. Bull World Health Organ E-pub: 19 Apr 2016.”

Real time detection kit for Zika virus targeting the NS1 gene. The kit contains positive control, primers and probe for 50 reactions. Real time primers and probe were published by “Pyke AT, Daly MT, Cameron JN, Moore PR, Taylor CT, Hewitson GR, et al. Imported zika virus infection from the cook islands into australia, 2014. PLoS Curr. 2014;6.”

Real time detection kit for Zika virus targeting the NS1 gene. The kit contains positive control, primers and probe for 50 reactions. Real time primers and probe were published by “Corman VM, Rasche A, et al. Clinical comparison, standardization and optimization of Zika virus molecular detection Submitted]. Bull World Health Organ E-pub: 19 Apr 2016.”

Real time detection kit for Zika virus targeting the NS2b gene. The kit contains positive control, primers and probe for 50 reactions. Real time primers and probe were published by “Waggoner JJ, Pinsky BA. Zika Virus: Diagnostics for an Emerging Pandemic Threat. J Clin Microbiol. 2016 Feb 17.“