EVAg

Training in Coronavirus reverse genetics to adapt novel techniques (ISA method) to large RNA viruses and compare different technologies under BSL-3 conditions

The in vitro transcribed RNA is the control for the first-line screening assay termed E gene assay. The SARS-CoV full virus RNA is required to control the two confirmatory assays. Dislaimer: Import permit maybe be needed in your country for the SARS-CoV full virus RNA. If yes, please indicate so in your online enquiry.

IF-slides to detect anti-nCoV antibodies by Immunofluorescence assay. The slides were prepared by infecting Vero cells with the first Swedish SARS-CoV-2 isolate (SARS-CoV-2/01/human/2020/SWE). The virus is fully sequenced (Genebank accession: MT093571). The slides are fixated in acetone, air-dried and preserved at -80°C. IF-analysis tested positive with SARS-CoV-2 infected human sera.