EVAg

SARS-CoV-2 England 2020 isolate

Freeze-dried SARS-CoV-2 virus, strain UVE/SARS-CoV-2/2021/FR/7b (lineage B 1. 1 .7, ex UK) France 2021, complete genome sequence.

Culture supernatant of SARS-CoV-2, hCoV-19/Sweden/20-53846/2020 (lineage B 1. 1 .7, ex UK); complete genome sequence

Infectious cell culture supernatant containing SARS-CoV-2 ( hCoV-19/Netherlands/NoordHolland_10159/2021, South African variant). This strain is preserved in viral storage medium -80°C.

SARS-CoV-2 ex-SA/2021 isolate (lineage B.1.351)

Freeze-dried SARS-CoV-2 virus, isolate England/MIG457/2020 (lineage B.1.1.7), England 2020, complete genome. Please note this product can not be supplied to private entities

Infectious cell culture supernatant containing SARS-CoV-2 ( hCoV-19/Aruba_11401/2021, California variant, nextstrain clade CAL.20C, lineage B.1.429). This strain is preserved in viral storage medium -80°C.

Infectious cell culture supernatant containing SARS-Cov-2 (strain human/DEU/HH-1/2020). This strain is preserved under Viral Storage Medium -80C. To confirm its identity the virus has been completely sequenced

Panel consists of (cell-culture) RNA from different coronaviruses (HCoV-Nl63, HCoV-229E, HCoV-OC43, MERS-CoV and SARS-CoV) and is intended for use as control of specificity of molecular assays. This panel contains sufficient RNA for t least 5 experiments. "Disclaimer: Import permit may be needed in your country for SARS-CoV full virus RNA. If yes, please indicate so in your online enquiry". Product for Alphacoronavirus and Betacoronavirus. ------------------ NB: indicated price is only valid for non-profit laboratories. Commercial users will be contacted about pricing.

N-terminal domain of the MERS CoV nucleocapsid (1-164). Pure recombinant protein produced in E. coli. resuspended in HEPES buffer 10 mM pH 7.5, NaCl 300 mM.

In vitro transcribed RNA for a specific SARS-coronavirus real time PCR.

Infectious cell culture supernatant containing SARS-CoV-2 (strain hCoV-19/Netherlands/ZuidHolland_10004/2020, D614G (S)). This strain is preserved in viral storage medium -80°C.

N-terminal domain of the SARS-CoV-2 nucleprotein (1-180). Pure recombinant protein produced in E. coli and fused with a Avi tag and a Hexa-Hisitidine tag.

C-terminal domain of the SARS-CoV-2 nucleprotein (247-418). Pure recombinant protein produced in E. coli and fused with a Avi tag and a Hexa-Hisitidine tag.

Training in Coronavirus reverse genetics to adapt novel techniques (ISA method) to large RNA viruses and compare different technologies under BSL-3 conditions

The in vitro transcribed RNA is the control for the first-line screening assay termed E gene assay. The SARS-CoV full virus RNA is required to control the two confirmatory assays. Dislaimer: Import permit maybe be needed in your country for the SARS-CoV full virus RNA. If yes, please indicate so in your online enquiry.

IF-slides to detect anti-nCoV antibodies by Immunofluorescence assay. The slides were prepared by infecting Vero cells with the first Swedish SARS-CoV-2 isolate (SARS-CoV-2/01/human/2020/SWE). The virus is fully sequenced (Genebank accession: MT093571). The slides are fixated in acetone, air-dried and preserved at -80°C. IF-analysis tested positive with SARS-CoV-2 infected human sera.