Culture Supernatant. This Hazara virus is preserved under Viral Storage Medium -80C.Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been partly sequenced.
Grown from a source vial “501Y.V2.H2DELTA 25 Dec 2020 CAT#002” passaged in Vero E6 cells with DMEM & 2% FCS, at 48 hr (CPE+) flask freeze/thawed and clarified by low speed centrifugation. Stored as 1 mL aliquots.
Grown from a source vial “501Y.V2.H2DELTA 25 Dec 2020 CAT#001” passaged in Vero E6 cells with DMEM & 2% FCS, at 48 hr (CPE+) flask freeze/thawed and clarified by low speed centrifugation. Stored as 1 mL aliquots.
