Plasmid encoding the full-length anti-genome of Marburg virus strain Musoke under the control of the T7 polymerase for rescue experiments. The plasmid contains two genetic markers: insertion of a SacII restriction site in the GP gene (nt position 10511a to g silent mutation) and a silent mutation at nt position 9485 c toT in the GP gene resulting in the deletion of a KpnI restriction site
Original strain TRVL9760 was maintained through passage by intracerebral mouse inoculation. Total 9 passages. Inoculated mouse tissue was used to infect Vero cells. Virus stock is P1 on Vero cells.
Kyasanur forest disease virus - original isolate. This Kyasanur forest disease virus[KFD] is freeze dried. Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been partly sequenced.
Kyasanur forest disease virus - original isolate. This Kyasanur forest disease virus[KFD] is preserved under Viral Storage Medium -80C.Tests for the presence of mycoplasmae were negative.