A virus causing a serious disease of citrus, especially Kinnow mandarin, in India. The virus (an isolate named K1) was obtained in 1996 from a Kinnow mandarin tree showing bright yellow ringspots on mature leaves.
Unit: vial containing lyophilized pieces of leaves
Aeonium ringspot virus, RNA1 and RNA2 full-length cDNAs are cloned in plasmid pUC18, under the control of a T7 promoter, allowing for transcription of infectious RNAs.
Unit: 2 micrograms of each plasmid DNA, allowing for the inoculation of six host plants (Nicotiana benthamiana) after T7-driven transcription
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