Double-stranded DNA copy of a coxsackievirus A17 genome without the capsid-encoding region, cloned into a plasmid backbone downstream a T7 promoter. After in vitro transcription, the corresponding RNA can be transfected into cells to give rise to a replicon that is self-replicative and can express the non-structural virus proteins but is unable to produce infectious particles. The plasmid can be amplified in E. coli.
Infectious clone: double-stranded DNA copy of the genome of Coxsackievirus A17 strain 67591 downstream a T7 promoter in a plasmid backbone. In vitro transcribed RNA produces the virus upon transfection of permissive cells. The plasmid can be amplified in E. coli.
