EVAg

Real time detection kit for MERS CoV targeting the "ORF 1a" containing positive control, primers and probe.

Infectious cell culture supernatant containing SARS-CoV-2 (strain hCoV-19/Netherlands/ZuidHolland_10004/2020, D614G (S)). This strain is preserved in viral storage medium -80°C.

Lyophilized encapsidated RNA for SARS-CoV-2 (2019-nCoV) real time RT-PCR targeting region of the N (CUB) gene, for 100 Rxns (Target designed by Charité Universitätsmedizin Berlin). The encapsidated RNA has been stabilized to serve as positive control for extraction and real time RT-PCR. Extraction is mandatory before use in real time RT-PCR. SOPs in the attached pdf. For RUO (Research Use Only)

N-terminal domain of the SARS-CoV-2 nucleprotein (1-180). Pure recombinant protein produced in E. coli and fused with a Avi tag and a Hexa-Hisitidine tag.

C-terminal domain of the SARS-CoV-2 nucleprotein (247-418). Pure recombinant protein produced in E. coli and fused with a Avi tag and a Hexa-Hisitidine tag.

Training in Coronavirus reverse genetics to adapt novel techniques (ISA method) to large RNA viruses and compare different technologies under BSL-3 conditions

Lyophilized encapsidated RNA for SARS-CoV-2 (2019-nCoV) real time RT-PCR targeting region of the RdRp (IP2/IP4) gene, for 100 Rxns (Target designed by Institut Pasteur: https://www.who.int/docs/default-source/coronaviruse/real-time-rt-pcr-assays-for-the-detection-of-sars-cov-2-institut-pasteur-paris.pdf?sfvrsn=3662fcb6_2) The encapsidated RNA has been stabilized to serve as positive control for extraction and real time RT-PCR. Extraction is mandatory before use in real time RT-PCR. SOPs in the attached pdf. For RUO (Research Use Only)

The in vitro transcribed RNA is the control for the first-line screening assay termed E gene assay. The SARS-CoV full virus RNA is required to control the two confirmatory assays. Dislaimer: Import permit maybe be needed in your country for the SARS-CoV full virus RNA. If yes, please indicate so in your online enquiry.

Lyophilized encapsidated RNA for SARS-CoV-2 (2019-nCoV) real time RT-PCR targeting region of the RdRp gene, for 25 Rxns (Target designed by Charité Universitätsmedizin Berlin). The encapsidated RNA has been stabilized to serve as positive control for extraction and real time RT-PCR. Extraction is mandatory before use in real time RT-PCR. SOPs in the attached pdf. For RUO (Research Use Only)

Lyophilized encapsidated RNA for SARS-CoV-2 (2019-nCoV) real time RT-PCR targeting region of the E gene, for 100 Rxns (Target designed by Charité Universitätsmedizin Berlin ; https://www.who.int/docs/default-source/coronaviruse/wuhan-virus-assay-v1991527e5122341d99287a1b17c111902.pdf). The encapsidated RNA has been stabilized to serve as positive control for extraction and real time RT-PCR. Extraction is mandatory before use in real time RT-PCR. SOPs in the attached pdf. For RUO (Research Use Only)

IF-slides to detect anti-nCoV antibodies by Immunofluorescence assay. The slides were prepared by infecting Vero cells with the first Swedish SARS-CoV-2 isolate (SARS-CoV-2/01/human/2020/SWE). The virus is fully sequenced (Genebank accession: MT093571). The slides are fixated in acetone, air-dried and preserved at -80°C. IF-analysis tested positive with SARS-CoV-2 infected human sera.

In this 2-day course the basic principles of routine virus neutralization and ELISA assays will be addressed by a combination of lectures, demonstration and hands-on training. Special attention will be given to quality control and assay validation. Topics include: cell culture, biosafety procedures, preparation of sera and virus stocks, ELISA and microarray formats, different virus neutralization tests using pseudotyped viruses and wildtype viruses, immunofluorescence staining of cells, calculation of titers, assay validation and quality control.

Anti-Middle East respiratory syndrome-related coronavirus NP protein polyclonal rabbit sera

Hybridoma cell culture supernatant containing anti-PEDV antibodies; targeted antigen: spike protein; clone: 4H4B1; immunization antigen: PEDV strain "CV777"; IgG subtype: IgG2a

Hybridoma cell culture supernatant containing anti-PEDV antibodies; targeted antigen: matrix protein; clone: 2C3B2 ; immunization antigen: PEDV strain "CV777"; IgG subtype: IgG2b

Hybridoma cell culture supernatant containing anti-PEDV antibodies; targeted antigen: NP; clone: 1B6A3 ; immunization antigen: PEDV strain "CV777"; IgG subtype: IgG2b