Aeonium ringspot virus, RNA1 and RNA2 full-length cDNAs are cloned in plasmid pUC18, under the control of a T7 promoter, allowing for transcription of infectious RNAs.
Unit: 2 micrograms of each plasmid DNA, allowing for the inoculation of six host plants (Nicotiana benthamiana) after T7-driven transcription
Recombinant hemagglutinin-esterase of Bovine coronavirus strain L9 (amino acids 17 - 392). The protein was expressed in Expi293 cells and Streptactin-purified. It is equipped with a c-terminal SpyTag (RGVPHIVMVDAYKRYK) and a single StrepTag
E2 glycoprotein of the Norway rat pestivirus isolate NrPV/NYC-D23; amino acids 803 - 1150 of the polyprotein. C-terminally, a single StrepTag was added. The protein was expressed in Expi293 cells and purified by Streptactin affinity chromatography.
