- Tagged as
- - Diagnostic reagent
- - Derived product
- - Antibody or Hybridoma
- - Antibody
Produced by
: ERASMUS-MC Shipping From
: Rotterdam - NL
Product Description
Ref-SKU:
010A-05018 Human monoclonal recombinant IgG1 targeting the S2 domain of HCoV-OC43 Spike domain, as determined by ELISA.
Product Risk Group:
Information on the related virus
ICTV Taxonomy:
Virus name:
Product type:
Antibody
Targeted antigen:
Virus
Antibody type:
Monoclonal
Antibody purified by affinity:
Yes
Production system:
Human HCoV-OC43 Spike specific B-cells were identified through culturing at limiting dilution. Heavy and Light variable sequences were amplified. After amplification the variable regions were cloned into Heavy and Light pFUSEss expression plasmids. The antibody was expressed by co-transfecting 293T cells with both plasmids. Secreted antibody was subsequently affinity purified and concentrated.
Antibody characterization method(s):
ELISA
Information on characterization:
The antibody showed reactivity towards HCoV-OC43 S-trimer (Sino Biological; 40607-V08B) and S2 domain (Sino Biological; 40607-V08B1). Antibody showed high affinity for S2 as determined by ELISA.
Specificity documented:
Yes
Virus host type:
Antibody specificity:
This antibody is specific for the S2 domain of spike protein of HCoV-OC43 virus.
Previous Name or Taxonomy:
Shipping conditions:
IATA Classification: