LOCUS       Exported                4459 bp DNA     circular SYN 13-SEP-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 13, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
COMMENT     Homologous recombination reaction between [pTIT.txt] as a target 
            sequence  and [PCR(N.xdna] as an inserted sequence.
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    695..2074
                     /label=Insert from PCR(N.xdna
                     /note="note=""Fragment inserted from PCR(N.xdna into 
                     pTIT.txt by homologous recombination"
ORIGIN
        1 tttcgcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
       61 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
      121 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgcgcgc gtaatacgac
      181 tcactatagg gcgaattaat tccggttatt ttccaccata ttgccgtctt ttggcaatgt
      241 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
      301 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
      361 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
      421 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
      481 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
      541 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
      601 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg
      661 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aataccatgg atgccgacaa
      721 gattgtattc aaagtcaata atcaggtggt ctccttgaag ccagagatta tcgtggatca
      781 gtatgagtac aaatacccag ctatcaagga cttgaagaaa cctagtataa ctctagggaa
      841 ggctcctgac ctgaataaag catacaagtc agtcctgtct ggcatgaacg ctgccaagct
      901 tgatcctgat gatgtgtgtt cctacttggc agctgcaatg caattctttg agggatcatg
      961 ccctgaagac tggaccagtt atggaatctt gattgcacga aaaggagaca agatcactcc
     1021 tgactctctt gtagagataa aacgtactga tgtagaaggt aattgggcac tgacgggagg
     1081 tatggaagtg acgagaracc ccaccgttgc tgaacatgca tctttagttg gtcttctctt
     1141 gagtctatat aggttgagca aaatatcggg gcaaaacact ggcaactata agacaaacat
     1201 tgcagacagg atagagcaga tttttgagac tgcccccttt gtaaagatcg tagaacacca
     1261 tactctaatg acaactcaca agatgtgcgc taattggagt accatcccga actttagatt
     1321 cttagctgga acctacgaca tgtttttctc tcggattgag catttgtact cagctataag
     1381 agtgggcaca gttgtcactg cttatgaaga ctgctcaggg ttggtatcgt tcacagggtt
     1441 cataaagcaa ataaatctca ccgcgagaga ggcaatccta tatttcttcc acaagaactt
     1501 tgaggaagaa ataagaagaa tgttcgaacc agggcaagaa actgcagtcc ctcactccta
     1561 tttcatccac ttccgctcat tgggcctgag tggaaagtct ccctattcat cgaatgcagt
     1621 tggtcatgtg ttcaatctca ttcactttgt cggatgttat atgggtcagg tcaggtctct
     1681 taatgccacg gttattgccg cttgtgcccc ccatgaaatg tcagttctcg gaggctattt
     1741 gggagaggag ttttttggaa aggggacgtt tgaaagacga ttctttaggg acgagaagga
     1801 acttcaggag tatgaggcag ctgagctgat gaagactgac gtagcactgg cagacgacgg
     1861 aaccgtcaat tctgatgacg aggattactt ctccggtgag acgaggagcc ctgaggccgt
     1921 ctatactcga atcatgatga acggaggtcg actaaagaga tcacacataa ggagatatgt
     1981 tgccgtcagt tctaaccatc aagcccgccc aaactcattt gccgagtttc taagcaagac
     2041 atattcaagt gactcgtaaa tcgaattcct gcagcccggg ggatccggct gctaacaaag
     2101 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
     2161 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatcca
     2221 ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg
     2281 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     2341 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     2401 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     2461 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     2521 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     2581 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     2641 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     2701 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2761 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2821 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2881 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     2941 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3001 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3061 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3121 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     3181 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3241 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3301 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3361 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3421 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3481 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     3541 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     3601 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     3661 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     3721 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     3781 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     3841 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     3901 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     3961 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4021 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4081 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4141 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4201 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4261 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4321 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4381 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     4441 ccccgaaaag tgccacctg
//
