LOCUS       INP135       12832 bp DNA     circular SYN 22-MAR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 22, 2024 from SnapGene 7.1.2
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     source          1..12832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) of HIV-1"
     misc_feature    682..806
                     /label=HIV-1 Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.2"
                     /note="/other=misc_feature"
     CDS             790..2292
                     /codon_start=1
                     /label=HIV-1 gag
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=98.3"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQDPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2085..5096
                     /codon_start=1
                     /label=HIV-1 pol
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     SDRQGTVSFSFPQITLWQRPIVTIKIGGQLKEALLDTGADDTVLEEVNLPGRWKPKMIG
                     GIGGFIKVRQYDQIPIEICGHKVIGTVLVGPTPANIIGRNLMTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     CDS             2253..2549
                     /codon_start=1
                     /label=protease
                     /label=pol protease
                     /note="/created_by=public1"
                     /translation="PQITLWQRPIVTIKIGGQLKEALLDTGADDTVLEEVNLPGRWKPK
                     MIGGIGGFIKVRQYDQIPIEICGHKVIGTVLVGPTPANIIGRNLMTQIGCTLNF"
     gene            2549..4029
                     /label=RT
                     /note="/Color=#ff5555"
                     /note="/created_by=public1"
     gene            4030..4389
                     /label=RNAseH
                     /note="/Color=#ffddff"
                     /note="/created_by=public1"
     gene            4390..5093
                     /label=INT
                     /note="/Color=#bbbbff"
                     /note="/created_by=public1"
     CDS             5039..5615
                     /label=vif
     CDS             5558..5845
                     /label=vpr
     CDS             5829..6044
                     /label=tat exon 1
     CDS             5968..6042
                     /label=rev exon 1
     CDS             6060..6302
                     /label=vpu
     CDS             6304..7743
                     /codon_start=1
                     /label=gp120
                     /note="gp120 Env coding sequence"
                     /translation="*CYRKIVGHSLLWGTCVEGSNHHSILCIRC*SI*YRGT*CLGHTC
                     LCTHRPQPTRSSIGKCDRKF*HVEK*HGRTDA*GYNQFMGSKPKAMCKINPTLC*FKVH
                     *FEE*Y*YQ***RENDNGERRDKKLLFQYQHKHKR*GAERICILL*T*YSTNR*YQL*V
                     DKL*HLSHYTGLSKGIL*ANSHTLLCPGWFCDSKM***DVQWNRTMYKCQHSTMYTWNQ
                     ASSINSTAVKWQSSRRRCSN*ICQFHRQC*NHNSTAEHICRN*LYKTQQQYKKKYPYPE
                     GTRESICYNRKNRKYETSTL*H**SKMECHFKTDS*QIKRTIWK**NNNL*AILRRGPR
                     NCNAQF*LWRGIFPL*FNTTV**YLV**YLEY*RVK*H*RK*HNHTPMQNKTIYKHVAG
                     SRKSNVCPSHQWTN*MFIKYYWAAINKRWW**QQWVRDLQTWRRRYEGQLEK*II*I*S
                     SKN*TIRSSTHQGKEKSGAERK"
     gene            7099..7207
                     /label=V3
                     /note="/Color=#3333ff"
                     /note="/created_by=public1"
     misc_feature    7759..7991
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8176..8220
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8302..8639
                     /codon_start=1
                     /label=rev exon 2
                     /translation="IVFAVLSIVNRVRQGYSPLSFQTHLPIPRGPDRPEGIEEEGGERD
                     RDRSIRLVNGSLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALKYWWNLL
                     QYWSQELK"
     CDS             8369..8410
                     /label=tat exon 2
     CDS             8786..9103
                     /label=nef
     LTR             9075..9708
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     rep_origin      10599..11187
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(join(11358..12149,12150..12218))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        12219..12323
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             complement(12660..12726)
                     /codon_start=1
                     /label=lacZ-alpha
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=38.5"
                     /note="/other=CDS"
                     /translation="LA**RRGPHRSPFPTVAQPEWR"
ORIGIN
        1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
      121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
      181 atgaaggaga gaacaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
      241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
      301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
      361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga taaatgggaa
      841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca
      901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt
      961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc
     1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa
     1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac
     1201 ctccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatgc atgggtaaaa
     1261 gtagtagaag agaaggcttt cagcccagaa gtaataccca tgttttcagc attatcagaa
     1321 ggagccaccc cacaagattt aaataccatg ctaaacacag tggggggaca tcaagcagcc
     1381 atgcaaatgt taaaagagac catcaatgag gaagctgcag aatgggatag attgcatcca
     1441 gtgcatgcag ggcctattgc accaggccag atgagagaac caaggggaag tgacatagca
     1501 ggaactacta gtacccttca ggaacaaata ggatggatga cacataatcc acctatccca
     1561 gtaggagaaa tctataaaag atggataatc ctgggattaa ataaaatagt aagaatgtat
     1621 agccctacca gcattctgga cataagacaa ggaccaaagg aaccctttag agactatgta
     1681 gaccgattct ataaaactct aagagccgag caagcttcac aagaggtaaa aaattggatg
     1741 acagaaacct tgttggtcca aaatgcgaac ccagattgta agactatttt aaaagcattg
     1801 ggaccaggag cgacactaga agaaatgatg acagcatgtc agggagtggg gggacccggc
     1861 cataaagcaa gagttttggc tgaagcaatg agccaagtaa caaatccagc taccataatg
     1921 atacagaaag gcaattttag gaaccaaaga aagactgtta agtgtttcaa ttgtggcaaa
     1981 gaagggcaca tagccaaaaa ttgcagggcc cctaggaaaa agggctgttg gaaatgtgga
     2041 aaggaaggac accaaatgaa agattgtact gagagacagg ctaatttttt agggaagatc
     2101 tggccttccc acaagggaag gccagggaat tttcttcaga gcagaccaga gccaacagcc
     2161 ccaccagaag agagcttcag gtttggggaa gagacaacaa ctccctctca gaagcaggat
     2221 ccgatagaca aggaactgta tcctttagct tccctcagat cactctttgg cagcgaccca
     2281 tcgtcacaat aaagataggg gggcaattaa aggaagctct attagataca ggagcagatg
     2341 atacagtatt agaagaagtg aatttgccag gaagatggaa accaaaaatg atagggggaa
     2401 ttggaggttt tatcaaagta agacagtatg atcagatacc catagaaatc tgtggacata
     2461 aagttatagg tacagtatta gtaggaccta cacctgccaa cataattgga agaaatctga
     2521 tgactcagat tggctgcact ttaaattttc ccattagtcc tattgagact gtaccagtaa
     2581 aattaaagcc cgggatggat ggcccaaaag ttaaacaatg gccattgaca gaagaaaaaa
     2641 taaaagcatt agtagaaatt tgtacagaaa tggaaaagga aggaaaaatt tcaaaaattg
     2701 ggcctgaaaa tccatacaat actccagtat ttgccataaa gaaaaaagac agtactaaat
     2761 ggagaaaatt agtagatttc agagaactta ataagagaac tcaagatttc tgggaagttc
     2821 aattaggaat accacatcct gcagggttaa aacagaaaaa atcagtaaca gtactggatg
     2881 tgggcgatgc atatttttca gttcccttag ataaagactt caggaagtat actgcattta
     2941 ccatacctag tataaacaat gagacaccag ggattagata tcagtacaat gtgcttccac
     3001 agggatggaa aggatcacca gcaatattcc agtgtagcat gacaaaaatc ttagagcctt
     3061 ttagaaaaca aaatccagac atagtcatct atcaatacat ggatgatttg tatgtaggat
     3121 ctgacttaga aatagggcag catagaacaa aaatagagga actgagacaa catctgttga
     3181 ggtggggatt taccacacca gacaaaaaac atcagaaaga acctccattc ctttggatgg
     3241 gttatgaact ccatcctgat aaatggacag tacagcctat agtgctgcca gaaaaggaca
     3301 gctggactgt caatgacata cagaaattag tgggaaaatt gaattgggca agtcagattt
     3361 atgcagggat taaagtaagg caattatgta aacttcttag gggaaccaaa gcactaacag
     3421 aagtagtacc actaacagaa gaagcagagc tagaactggc agaaaacagg gagattctaa
     3481 aagaaccggt acatggagtg tattatgacc catcaaaaga cttaatagca gaaatacaga
     3541 agcaggggca aggccaatgg acatatcaaa tttatcaaga gccatttaaa aatctgaaaa
     3601 caggaaagta tgcaagaatg aagggtgccc acactaatga tgtgaaacaa ttaacagagg
     3661 cagtacaaaa aatagccaca gaaagcatag taatatgggg aaagactcct aaatttaaat
     3721 tacccataca aaaggaaaca tgggaagcat ggtggacaga gtattggcaa gccacctgga
     3781 ttcctgagtg ggagtttgtc aatacccctc ccttagtgaa gttatggtac cagttagaga
     3841 aagaacccat aataggagca gaaactttct atgtagatgg ggcagccaat agggaaacta
     3901 aattaggaaa agcaggatat gtaactgaca gaggaagaca aaaagttgtc cccctaacgg
     3961 acacaacaaa tcagaagact gagttacaag caattcatct agctttgcag gattcgggat
     4021 tagaagtaaa catagtgaca gactcacaat atgcattggg aatcattcaa gcacaaccag
     4081 ataagagtga atcagagtta gtcagtcaaa taatagagca gttaataaaa aaggaaaaag
     4141 tctacctggc atgggtacca gcacacaaag gaattggagg aaatgaacaa gtagataaat
     4201 tggtcagtgc tggaatcagg aaagtactat ttttagatgg aatagataag gcccaagaag
     4261 aacatgagaa atatcacagt aattggagag caatggctag tgattttaac ctaccacctg
     4321 tagtagcaaa agaaatagta gccagctgtg ataaatgtca gctaaaaggg gaagccatgc
     4381 atggacaagt agactgtagc ccaggaatat ggcagctaga ttgtacacat ttagaaggaa
     4441 aagttatctt ggtagcagtt catgtagcca gtggatatat agaagcagaa gtaattccag
     4501 cagagacagg gcaagaaaca gcatacttcc tcttaaaatt agcaggaaga tggccagtaa
     4561 aaacagtaca tacagacaat ggcagcaatt tcaccagtac tacagttaag gccgcctgtt
     4621 ggtgggcggg gatcaagcag gaatttggca ttccctacaa tccccaaagt caaggagtaa
     4681 tagaatctat gaataaagaa ttaaagaaaa ttataggaca ggtaagagat caggctgaac
     4741 atcttaagac agcagtacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     4801 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     4861 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     4921 gagatccagt ttggaaagga ccagcaaagc tcctctggaa aggtgaaggg gcagtagtaa
     4981 tacaagataa tagtgacata aaagtagtgc caagaagaaa agcaaagatc atcagggatt
     5041 atggaaaaca gatggcaggt gatgattgtg tggcaagtag acaggatgag gattaacaca
     5101 tggaaaagat tagtaaaaca ccatatgtat atttcaagga aagctaagga ctggttttat
     5161 agacatcact atgaaagtac taatccaaaa ataagttcag aagtacacat cccactaggg
     5221 gatgctaaat tagtaataac aacatattgg ggtctgcata caggagaaag agactggcat
     5281 ttgggtcagg gagtctccat agaatggagg aaaaagagat atagcacaca agtagaccct
     5341 gacctagcag accaactaat tcatctgcac tattttgatt gtttttcaga atctgctata
     5401 agaaatacca tattaggacg tatagttagt cctaggtgtg aatatcaagc aggacataac
     5461 aaggtaggat ctctacagta cttggcacta gcagcattaa taaaaccaaa acagataaag
     5521 ccacctttgc ctagtgttag gaaactgaca gaggacagat ggaacaagcc ccagaagacc
     5581 aagggccaca gagggagcca tacaatgaat ggacactaga gcttttagag gaacttaaga
     5641 gtgaagctgt tagacatttt cctaggatat ggctccataa cttaggacaa catatctatg
     5701 aaacttacgg ggatacttgg gcaggagtgg aagccataat aagaattctg caacaactgc
     5761 tgtttatcca tttcagaatt gggtgtcgac atagcagaat aggcgttact cgacagagga
     5821 gagcaagaaa tggagccagt agatcctaga ctagagccct ggaagcatcc aggaagtcag
     5881 cctaaaactg cttgtaccaa ttgctattgt aaaaagtgtt gctttcattg ccaagtttgt
     5941 ttcatgacaa aagccttagg catctcctat ggcaggaaga agcggagaca gcgacgaaga
     6001 gctcatcaga acagtcagac tcatcaagct tctctatcaa agcagtaagt agtacatgta
     6061 atgcaaccta taatagtagc aatagtagca ttagtagtag caataataat agcaatagtt
     6121 gtgtggtcca tagtaatcat agaatatagg aaaatattaa gacaaagaaa aatagacagg
     6181 ttaattgata gactaataga aagagcagaa gacagtggca atgagagtga aggagaagta
     6241 tcagcacttg tggagatggg ggtggaaatg gggcaccatg ctccttggga tattgatgat
     6301 ttgtagtgct acagaaaaat tgtgggtcac agtctattat ggggtacctg tgtggaagga
     6361 agcaaccacc actctatttt gtgcatcaga tgctaaagca tatgatacag aggtacataa
     6421 tgtttgggcc acacatgcct gtgtacccac agaccccaac ccacaagaag tagtattggt
     6481 aaatgtgaca gaaaatttta acatgtggaa aaatgacatg gtagaacaga tgcatgagga
     6541 tataatcagt ttatgggatc aaagcctaaa gccatgtgta aaattaaccc cactctgtgt
     6601 tagtttaaag tgcactgatt tgaagaatga tactaatacc aatagtagta gcgggagaat
     6661 gataatggag aaaggagaga taaaaaactg ctctttcaat atcagcacaa gcataagaga
     6721 taaggtgcag aaagaatatg cattctttta taaacttgat atagtaccaa tagataatac
     6781 cagctatagg ttgataagtt gtaacacctc agtcattaca caggcctgtc caaaggtatc
     6841 ctttgagcca attcccatac attattgtgc cccggctggt tttgcgattc taaaatgtaa
     6901 taataagacg ttcaatggaa caggaccatg tacaaatgtc agcacagtac aatgtacaca
     6961 tggaatcagg ccagtagtat caactcaact gctgttaaat ggcagtctag cagaagaaga
     7021 tgtagtaatt agatctgcca atttcacaga caatgctaaa accataatag tacagctgaa
     7081 cacatctgta gaaattaatt gtacaagacc caacaacaat acaagaaaaa gtatccgtat
     7141 ccagagggga ccagggagag catttgttac aataggaaaa ataggaaata tgagacaagc
     7201 acattgtaac attagtagag caaaatggaa tgccacttta aaacagatag ctagcaaatt
     7261 aagagaacaa tttggaaata ataaaacaat aatctttaag caatcctcag gaggggaccc
     7321 agaaattgta acgcacagtt ttaattgtgg aggggaattt ttccactgta attcaacaca
     7381 actgtttaat agtacttggt ttaatagtac ttggagtact gaagggtcaa ataacactga
     7441 aggaagtgac acaatcacac tcccatgcag aataaaacaa tttataaaca tgtggcagga
     7501 agtaggaaaa gcaatgtatg cccctcccat cagtggacaa attagatgtt catcaaatat
     7561 tactgggctg ctattaacaa gagatggtgg taataacaac aatgggtccg agatcttcag
     7621 acctggagga ggcgatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     7681 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     7741 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag aggaagcact
     7801 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tgatatagtg
     7861 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     7921 gtctggggca tcaaacagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     7981 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     8041 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaataacat gacctggatg
     8101 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     8161 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     8221 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     8281 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     8341 cagggatatt caccattatc gtttcagacc cacctcccaa tcccgagggg acccgacagg
     8401 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     8461 aacggatcat tagcacttat ctgggacgat ctgcggagcc tgtgcctctt cagctaccac
     8521 cgcttgagag acttactctt gattgtaacg aggattgtgg aacttctggg acgcaggggg
     8581 tgggaagccc tcaaatattg gtggaatctc ctacagtatt ggagtcagga actaaagaat
     8641 agtgctgtta acttgctcaa tgccacagcc atagcagtag ctgaggggac agatagggtt
     8701 atagaagtat tacaagcagc ttatagagct attcgccaca tacctagaag aataagacag
     8761 ggcttggaaa ggattttgct ataagatggg tggcaagtgg tcaaaaagta gtgtgattgg
     8821 atggcctgct gtaagggaaa gaatgagacg agctgagcca gcagcagatg gggtgggagc
     8881 agtatctcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaacaa
     8941 tgctgcttgt gcctggctag aagcacaaga ggaggaagag gtgggttttc cagtcacacc
     9001 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     9061 agaaaagggg ggactggaag ggctaattca ctcccaaaga agacaagata tccttgattt
     9121 gtggatttac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     9181 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     9241 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     9301 aatggatgac cctgagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
     9361 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgacatc gagcttgcta
     9421 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
     9481 gcgagccctc agatgctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
     9541 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     9601 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     9661 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcacc caggaggtag
     9721 aggttgcagt gagccaagat cgcgccactg cattccagcc tgggcaagaa aacaagactg
     9781 tctaaaataa taataataag ttaagggtat taaatatatt tatacatgga ggtcataaaa
     9841 atatatatat ttgggctggg cgcagtggct cacacctgcg cccggccctt tgggaggccg
     9901 aggcaggtgg atcacctgag tttgggagtt ccagaccagc ctgaccaaca tggagaaacc
     9961 ccttctctgt gtatttttag tagattttat tttatgtgta ttttattcac aggtatttct
    10021 ggaaaactga aactgttttt cctctactct gataccacaa gaatcatcag cacagaggaa
    10081 gacttctgtg atcaaatgtg gtgggagagg gaggttttca ccagcacatg agcagtcagt
    10141 tctgccgcag actcggcggg tgtccttcgg ttcagttcca acaccgcctg cctggagaga
    10201 ggtcagacca cagggtgagg gctcagtccc caagacataa acacccaaga cataaacacc
    10261 caagacataa acacccaaca ggtccacccc gcctgctgcc caggcagagc cgattcacca
    10321 agacgggaat taggatagag aaagagtaag tcacacagag ccctgcatta atgaatcggc
    10381 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
    10441 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    10501 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
    10561 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    10621 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    10681 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    10741 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    10801 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    10861 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    10921 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    10981 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
    11041 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    11101 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    11161 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    11221 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    11281 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    11341 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    11401 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    11461 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    11521 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    11581 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    11641 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    11701 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    11761 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    11821 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    11881 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    11941 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    12001 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    12061 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    12121 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    12181 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    12241 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    12301 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
    12361 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
    12421 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
    12481 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
    12541 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
    12601 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
    12661 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
    12721 cgccaggcat cagagtcctt ggtgtggagg gagggaccag cgcagcttcc agccatccac
    12781 ctgatgaaca gaacctaggg aaagccccag ttctacttac accaggaaag gc
//
