- Tagged as
- - Antigen
- - Diagnostic reagent
- - Virus
- - Cultivable virus
Produced by
: ERASMUS-MC Shipping From
: Rotterdam - NL
Product Description
Ref-SKU:
011V-02815 This clone belongs to a unique set of HIV-2 biological clones from West African immigrants in The Netherlands. Clones were obtained from patients with non-progressive, aviremic or progressive, viremic HIV-2 infection. The HIV-2 biological clones were isolated after limiting dilution co-culture of patient PBMC or plasma with CD8-depleted PBMC from healthy blood bank donors.
The clone is replication competent and has been characterized for in vitro replication kinetics and co-receptor usage. Sequence is available in Genbank.
Product Risk Group:
ICTV Taxonomy:
Virus name:
Subspecies:
Strain:
Storage conditions:
Viral Storage Medium -80C
Sequencing:
Complete coding sequence
Infectivity:
Infectivity tested
Mycoplasmic content:
Not tested
GMO:
No
Biosafety restrictions:
BSL3
Virus host type:
department of Virology, Erasmus MC, Rotterdam, The Netherlands, Originator name or acronym: Erasmus MC, Viroscience lab
https://www.erasmusmc.nl/viroscience/
Infectivity Test:
virus production after co-culture of PBMC with CD8-depleted donor PBMC. Virus production in the supernatant was tested with HIV-2 viral RNA qPCR, plus TZA assay.
Production cell line:
Genbank reference:
Virus Cultivability:
Passage:
Low passage number (estimated 3 cell free passages)
Identification technique:
HIV-2 qPCR on culture supernatant
Shipping conditions:
IATA Classification:
Information about the collection of the virus
Recombinant product:
No
Biological material origin:
Natural origin
Collection date:
Saturday, 1 June, 2002
Country of collection:
Suspected epidemiological origin :
Isolation technique:
infection of donor PBMC
Isolation conditions:
infection of CD8 depleted donor PBMC
Clinical information:
progressive HIV-2 infection, plasma viral load >500 copies/ml, CD4 T cell count 10 cells/uL, cART naïve