Product Description
Ref-SKU:
016N-05980 Plasmid encoding the full-length anti-genome of Marburg virus strain Musoke under the control of the T7 polymerase for rescue experiments. The plasmid contains two genetic markers: insertion of a SacII restriction site in the GP gene (nt position 10511a to g silent mutation) and a silent mutation at nt position 9485 c toT in the GP gene resulting in the deletion of a KpnI restriction site
Product Risk Group:
Information on the related virus
ICTV Taxonomy:
Virus name:
Isolate:
Cloned Product: Yes
Can it be used to produce GMO:
Yes
Biosafety restrictions:
the product can be used for the rescue of recombinant RG4 virus, it will be therfore supplied only to operating BSL4 laboratories
Region encompassed in this Product:
full-length anti-genome of Marburg virus Musoke
Plasmid:
Plasmid selection:
TAG:
Sequencing:
Fully sequenced
Sequence checked:
Yes
Mutations:
Contain mutations (no frameshift, no unexpected STOP codon)
Observed mutations:
Pos 9485 C>T in GP gene; Pos 10511 A>G in the GP gene silent genetic markers for recombinant virus
Genbank reference:
Identification technique:
sequencing and enzymatic restriction
Nucleic Acid preparation technique:
Quigen Maxi-prep Kit
Storage conditions:
TE Buffer -20C
Shipping conditions:
IATA Classification:
Virus host type:
Information about the origin of the product
Recombinant product:
This product is a recombinant product
Biological material origin:
Synthetic origin & Natural origin
Natural part identification: sequence from Marburg virus Musoke, isolated in Keny 1980
Natural part identification:
sequence from Marburg virus Musoke, isolated in Keny 1980
Genbank reference:
Collection date: BEFORE
Wednesday, 22 February, 2006
Country of collection:
Synthetic part identification: plasmid DNA for ampilification in bacteria
Synthetic part identification:
plasmid DNA for ampilification in bacteria
Genbank reference:
Modifications from the reference sequence(s):
The sequence(s) of this synthetic product has NOT been modified from the sequence listing provided as Genbank references