- Tagged as
- - Diagnostic reagent
- - Inactivated virus
- - Virus
- - Cultivable virus
Produced by
: UNIBAS Shipping From
: Basel - CH
Product Description
Ref-SKU:
007V-05545 This clonal virus is based on the Wuhan strain and carries a precise deletion of the viral E gene (Envelope). In its place, a functional GFP gene has been inserted.
The mutation renders the virus replication incompetent in the absence of a trans-complemented E function. The missing E protein can be provided by means of a SARS-CoV-2-susceptible VeroE6 derivatized cell line (in stock).
The produced virus can be used as a "single-cycle" virus, which is able to infect cells and produce all proteins (except E) for one cycle of virus production.
Product Risk Group:
ICTV Taxonomy:
Genotype:
Storage conditions:
Viral Storage Medium -20C
Sequencing:
Complete genome
Infectivity:
Infectivity tested and quantified
Mycoplasmic content:
Mycoplasma free
Special feature:
GMO:
Yes
Biosafety restrictions:
This virus has been granted BSL-2 level status in Switzerland, since it cannot replicate in the absence of supplementing helper cell functions (provision of E in trans)
Virus host type:
Production cell line:
Genbank reference:
Virus Cultivability:
Passage:
1
Shipping conditions:
IATA Classification:
Information about the collection of the virus
Recombinant product:
No
Biological material origin:
Synthetic origin
Modifications from the reference sequence(s):
The sequence(s) of this synthetic product has been modified from the sequence listing provided as Genbank references
Description of modification(s) made from the reference sequence(s):
specific deletion of the viral E gene; insertion of a functional GFP gene in the position of the E-gene.