- Tagged as
- - Diagnostic reagent
- - Derived product
- - Antibody or Hybridoma
- - Antibody
Produced by
: ERASMUS-MC Shipping From
: Rotterdam - NL
Product Description
Ref-SKU:
010A-04961 Human monoclonal recombinant IgG1 targeting the RBD domain of SARS-CoV-2 Spike domain of the wild type strain, as determined by ELISA.
Product Risk Group:
Information on the related virus
ICTV Taxonomy:
Virus name:
Product type:
Antibody
Targeted antigen:
Protein
Antibody type:
Monoclonal
Antibody purified by affinity:
Yes
Production system:
Human SARS-CoV-2 Spike specific single B-cells are FACS sorted. After amplification the variable regions are cloned into Heavy and Light pFUSEss plasmids. The antibody is brought to expression by co-transfecting 293T cells and subsequently affinity purified and concentrated.
Antibody characterization method(s):
ELISA
Information on characterization:
SARS-CoV-2 Spike antigen specific binding is determined using ELISA: Secto (Sino Biological; 40589-V08H8) positive, S1 (Sino Biological; 40591-V08H3) positive and RBD (Sino Biological; 40592-V08H) positive. Antibody showed high affinity for RBD as determined by ELISA. Binding to SARS-CoV2 was also verified on protein array and no cross-reactivity to hCoV-OC43, hCoV-NL63, hCoV-HKU1, hCoV-229E was detected. Binding to SARS-CoV2 variants was also verified on protein array and reactivity to WT, Delta, Omicron BA.1, Omicron BA.5 was detected.
Specificity documented:
Yes
Virus host type:
Previous Name or Taxonomy:
Shipping conditions:
IATA Classification: